Middlesex -- U.S. County in Virginia Population (2000): 9932 Housing Units (2000): 6362 Land area (2000): 130.298269 sq. miles (337.470952 sq. km) Water area (2000): 80.455402 sq. miles (208.378526 sq. km) Total area (2000): 210.753671 sq. miles (545.849478 sq. km) Located within: Virginia (VA), FIPS 51 Location: 37.595718 N, 76.489133 W Headwords: Middlesex Middlesex, VA Middlesex County Middlesex County, VA